Effect of omeprazole on ascorbate free radical formation.
نویسندگان
چکیده
In order to study the effect of omeprazole on the K/[H+] value of ascorbic acid (K = the ascorbic acid-ascorbic free radical equilibrium constant), changes of the concentration of ascorbate free radical in guinea pig sera were examined after intraperitoneal administration of omeprazole. Furthermore, to see the in vitro effect of omeprazole on ascorbate free radical, changes were examined in ascorbate solutions after addition of omeprazole. It was found that the K/[H+] value in the serum increased significantly after administration of omeprazole and also that the drug amplified the electron spin resonance (ESR) signal intensity of ascorbate free radical in vitro. These results suggested that omeprazole acts like oxygen radicals.
منابع مشابه
Hydrogen peroxide and hydroxyl radical formation by methylene blue in the presence of ascorbic acid.
Using ESR we have demonstrated the formation of the ascorbate free radical from sodium ascorbate, methylene blue and light. In oxygen uptake experiments we have observed the production of hydrogen peroxide while spin trapping experiments have revealed the iron catalyzed production of the hydroxyl free radical in this system. The presence of this highly reactive radical suggests that it could be...
متن کاملDevelopment of a Method for measuring Reactive Oxygen Radicals Levels In Vitro and Study the Effects of Vitamin C and E on Radical Production Reaction
Background: Free radicals and reactive oxygen species(ROS) are the most important factors in formation of oxidative stress reaction. Now, radical damage has been suggested to contribute to a wide variety of diseases such as Alzheimer, atherosclerosis and cancer. Transition metal ions in the presence of the various biomolecules produce these active compounds. The aim of this study is introducing...
متن کاملInhibition of astrocyte glutamate uptake by reactive oxygen species: role of antioxidant enzymes.
BACKGROUND The recent literature suggests that free radicals and reactive oxygen species may account for many pathologies, including those of the nervous system. MATERIALS AND METHODS The influence of various reactive oxygen species on the rate of glutamate uptake by astrocytes was investigated on monolayers of primary cultures of mouse cortical astrocytes. RESULTS Hydrogen peroxide and per...
متن کاملThe ascorbate-driven reduction of extracellular ascorbate free radical by the erythrocyte is an electrogenic process.
Erythrocytes can reduce extracellular ascorbate free radicals by a plasma membrane redox system using intracellular ascorbate as an electron donor. In order to test whether the redox system has electrogenic properties, we studied the effect of ascorbate free radical reduction on the membrane potential of the cells using the fluorescent dye 3,3'-dipropylthiadicarbocyanine iodide. It was found th...
متن کاملEvaluation of Antioxidant Effect of Various Extracts of Leucas Aspera in Vitro
Lamiaceae herbs known for their culinary properties are a rich source of potentially health-beneficial antioxidant polyphenols. The objective of this investigation was to evaluate and prove the in vitro antioxidant activity of five different extracts of aerial parts of the plant Leucas aspera, belonging to the family Lamiaceae on different models namely the DPPH free radical scavenging assay, F...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Tohoku journal of experimental medicine
دوره 167 3 شماره
صفحات -
تاریخ انتشار 1992